Amidolytic properties of single-chain activated Hageman factor.
نویسندگان
چکیده
منابع مشابه
Amidolytic properties of single-chain activated Hageman factor.
Activation of Hageman factor (Factor XII) upon exposure to negatively charged agents has been attributed to proteolytic cleavage of this molecule. To examine this question, purified Hageman factor was exposed to Sephadex gels to which ellagic acid had been adsorbed. Such Hageman factor, separated from the gels and studied in the fluid phase, was amidolytic. Nonetheless, no cleavage of Hageman f...
متن کاملStudies on the inhibition of ellagic acid-activated Hageman factor (factor XII) and Hageman factor fragments.
Hageman factor (HF, factor XII) that has been exposed to Sephadex-ellagic acid gels is a single-chain species (HFea) with amidolytic properties for the synthetic substrate H-D-phenylalanyl-L-pipecolyl-L-arginine p-nitroanilide. Earlier we reported that amidolysis was suppressed by incubation of HFea with specific antiserum. The present study provides additional evidence that the amidolytic prop...
متن کامل798 Hageman Factor Activation
Bacterial lipopolysaccharides (LPS) I or endotoxins elicit a variety of host responses, the combination of which result in the deposition of fibrin in capillaries and disseminated intravascular coagulation (DIC). These various host responses to endotoxins have recently been summarized (1) and include: (a) aggregation of platelets and release of platelet factor 3; (b) activation of Hageman facto...
متن کاملSubstrates of Hageman Factor
Unactivated partial thromboplastin antecedent (PTA) has been purified by sequential chromatography of plasma on quaternary aminoethyl Sephadex, sulphoprophyl Sephadex, Sephadex G-150, and passage over an anti-IgG immunoadsorbant. The preparation gave a single band after alkaline disc gel electrophoresis, sodium dodecyl sulfate (SDS) gel electrophoresis and isoelectric focusing in acrylamide gel...
متن کاملEnzymatic activities of activated and zymogen forms of human Hageman factor (factor XII).
Pro-Phe-Arg chloromethylketone (PPACMK) at 5.26 microM inactivated the amidolytic activity of native human Hageman factor with an apparent first-order rate constant of 0.75 min-1. The activated forms of Hageman factor, Hfa and HFf, were also inactivated by PPACMK with rate constants 0.82 and 0.72 min-1. These numbers indicate that the activity detectable in native Hageman factor is due to conta...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1979
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.76.3.1461